Plasmid

Part:BBa_K2911001:Design

Designed by: Ryan Lambert   Group: iGEM19_USAFA   (2019-10-14)


Empty pSRKBB plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2049
    Illegal XbaI site found at 2067
    Illegal SpeI site found at 2356
    Illegal PstI site found at 2374
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2049
    Illegal SpeI site found at 2356
    Illegal PstI site found at 2374
    Illegal NotI site found at 2282
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2049
    Illegal BglII site found at 2206
    Illegal BamHI site found at 2212
    Illegal XhoI site found at 2290
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2049
    Illegal XbaI site found at 2067
    Illegal SpeI site found at 2356
    Illegal PstI site found at 2374
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2049
    Illegal XbaI site found at 2067
    Illegal SpeI site found at 2356
    Illegal PstI site found at 2374
    Illegal NgoMIV site found at 748
    Illegal NgoMIV site found at 1031
    Illegal NgoMIV site found at 4209
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5369
    Illegal SapI.rc site found at 597
    Illegal SapI.rc site found at 807
    Illegal SapI.rc site found at 1964


Design Notes

Plasmid works in both E. Coli and Rhodococcus.


Source

From Addgene. Derived from pSRK21.

References